Germany Journal

Document Type : Original Article


1 Pirogov Russian National Research Medical University, Moscow, Russia.

2 Institute for Biomedical Research, Association of Pharmaceutical Research and Development, Kyiv, Ukraine.


Background and purpose: Carbon tetrachloride (CCl4) has been applied to induce the toxicity and hepatic fibrosis. Natural antioxidants are known as efficient and safe treatments for hepatotoxicity compared with synthetic antioxidants. This study aimed to evaluate the effects of oral administration of pulegone in carbon tetrachloride-induced oxidative stress in Wistar rats.
Materials and Methods: Twenty rats were randomly assigned into four groups including control animals that received olive oil, Toxic control that administrated with 30% CCl4, Pulegone-20 & 30 that administrated with pulegone 20 mg/kg and 30 mg/kg, respectively, with in along to 30% CCl4. The liver levels of total cholesterol, triacylglycerides, phospholipids, superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), glutathione peroxidase (GPx), and vitamins C and E were evaluated.
Results: Administration of CCl4 increased levels of cholesterol, triacylglycerides and lipid oxidation, but it also reduced levels of phospholipids, SOD, CAT, GSH, GPx, and vitamins C and E (P<0.05). Oral administration of pulegone, especially in the higher levels, could reverse negative effects of CCl4 (P<0.05).
Conclusion: Using pulegone is recommended for liver protection due to its vital therapeutic antioxidant properties.