Background and purpose: Diethylnitrosamine (DENA) is one carcinogen toxin that is mainly found in the environment and can induce kidney injuries. The use of antioxidants can alleviate adverse effects of DENA on kidney. Seemingly, gamma oryzanol (GO), as an antioxidant, could alleviate adverse effects of GO on DENA. This study was conducted for first time to evaluate the effects of GO on DENA-induced oxidative stress in rat kidney.
Materials and Methods: Sixty male Wistar rats were divided into four groups, including: (1) negative control (lack of stress), (2) positive control (untreated rats with stress), (3 & 4) stressed rats treated with GO in the levels of 3000 and 6000 µg/kg. The serum concentrations of creatinine and urea, lipid peroxidation (LPO), reduced glutathione (GSH) level, and activities of the antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) were determined.
Results: The results showed that induction of stress with DENA increased the serum concentrations of creatinine and urea and LPO, but decreased the levels of GSH, SOD, CAT, GPx and GR (P<0.05). The treatment with GO in the both levels alleviated the effects of stress on LPO and antioxidant system (P<0.05).
Conclusion: In sum, GO could decreased adverse effects of stress on kidney parameters and it could be advised to use GO for decreasing stress.