Germany Journal

Document Type : Original Article


1 Department of Medical Research, Institute for Intelligent Research, Tbilisi, Georgia.

2 Semnan University of Medical Sciences, Semnan, Iran.


Background and purpose:  The use of natural antimicrobial agents can decrease the risk of infection during wound healing. Olive (Olea europaea L.) is anti-inflammatory agent that can be used in this way. This study was conducted for the first time to evaluate the effects of ointments prepared from olive leaf extract (OLE) on inflammatory gene expressions in infected wound healing process in mice model.
Materials and Methods:  We created two circular full-thickness wounds on the dorsal inter-scapular part of per mouse by a 5 mm biopsy punch and infected with Staphylococcus aureus and Pseudomonas aeruginosa. Following induction of infection, animals were treated with mupirocin (mupirocin group) and basal ointments containing 2.5% and 5% of the extract (2.5% & 5% OLE). A control group was also considered. We selected five mice per group and samples were collected at days 3, 7, and 14 for investigation of total bacterial count and gene expressions.
Results:  The results showed that control group significantly showed higher wound area, total bacterial count, and higher expressions of IL-1β and TNF-α (P<0.05) and lower expressions for IL-10 and TGF-β (P<0.05). The treatment with OLE could significantly decrease wound area, total bacterial count, expressions of IL-1β and TNF-α, and increase the expression of IL-10 and TGF-β (P<0.05).
Conclusion: In sum, ointments prepared from OLE could decrease total bacterial count, decrease inflammatory phase, and improve wound healing. We suggested to use the OLE for the treatment of wound healing in combination with synthetic agents.