en Original Research <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="background:white"><span cambria="" style="font-family:"><span style="color:#7030a0">Aims:</span></span></span></span></b><span style="font-size:9.0pt"><span style="background:white"><span cambria="" style="font-family:"> Natural antimicrobial agents can decrease the risk of infection during wound healing. <i>Olea europaea</i> L. is an anti-inflammatory agent that can be used this way. This study was conducted for the first time to evaluate the effects of ointments prepared from olive leaf extract on inflammatory gene expressions in a mouse model&#39;s infected wound healing process.</span></span></span><span style="font-size:9.0pt"><span style="background:white"><span style="font-family:"Cambria",serif"></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="background:white"><span cambria="" style="font-family:"><span style="color:#7030a0">Materials & Methods:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="background:white"><span cambria="" style="font-family:">We created two circular full-thickness wounds on the dorsal inter-scapular part of per mouse by a 5 mm biopsy punch and infected with Staphylococcus aureus and Pseudomonas aeruginosa. Following induction of infection, animals were treated with mupirocin (mupirocin group) and basal ointments containing 2.5% and 5% of the extract (2.5% & 5% OLE). A control group was also considered. We selected five mice per group, and samples were collected on days 3, 7, and 14 to investigate the total bacterial count and gene expressions.</span></span></span><span style="font-size:9.0pt"><span style="background:white"><span style="font-family:"Cambria",serif"></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="background:white"><span cambria="" style="font-family:"><span style="color:#7030a0">Findings:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="background:white"><span cambria="" style="font-family:">The control group significantly showed higher wound area, total bacterial count, and higher expressions of IL-1&beta; and TNF-&alpha; (p<0.05) and lower expressions for IL-10 and TGF-&beta; (p<0.05). The treatment with OLE could significantly decrease wound area, total bacterial count, and expressions of IL-1&beta; and TNF-&alpha; and increase the expression of IL-10 and TGF-&beta; (p<0.05).</span></span></span><span style="font-size:9.0pt"><span style="background:white"><span style="font-family:"Cambria",serif"></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span style="background:white"><span cambria="" style="font-family:"><span style="color:#7030a0">Conclusion:</span></span></span></span></b> <span style="font-size:9.0pt"><span style="background:white"><span cambria="" style="font-family:">Ointments prepared from OLE could decrease total bacterial count, decrease the inflammatory phase, and improve wound healing.</span></span></span></span></span></span> IL-1β,Inflammatory phase,Olea europaea,TNF-α,Wound healing, 63 67 http://gmedicine.de/browse.php?a_code=A-10-1-49&slc_lang=en&sid=2 F. Jazideh 10031947532846001319 10031947532846001319 No Department of Medical Research, Institute for Intelligent Research, Tbilisi, Georgia E. Tarkhnishvili 10031947532846001320 10031947532846001320 No Department of Medical Research, Institute for Intelligent Research, Tbilisi, Georgia S.E. Hashemi Feyzabadi 10031947532846001321 10031947532846001321 Yes Semnan University of Medical Sciences, Semnan, Iran