en <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span cambria="" style="font-family:"><span style="color:#7030a0">Aims:</span></span></span></b> <span style="font-size:9.0pt"><span cambria="" style="font-family:">Malaria is one of most important diseases especially in tropical regions. Flavonoids are known to have beneficial properties that could be profitable. This study aimed to evaluate the effects of nano-phytosomes of Quercetin on liver damages of mice infected with <i>Plasmodium berghei</i>.</span></span><span style="font-size:9.0pt"><span style="background:white"><span style="font-family:"Cambria",serif"></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span cambria="" style="font-family:"><span style="color:#7030a0">Materials & Methods:</span></span></span></b> <span style="font-size:9.0pt"><span cambria="" style="font-family:">A total of 60 male BALB/c mice were intra-peritoneally infected by administration of 106 <i>P. berghei</i>-infected RBCs. Animals were acclimatized for 7 days and divided into 5 groups; 0.9% isotonic saline (as negative control), saline treated (as positive control), 2mg/kg of Hydroxychloroquine sulfate treated for 4 days (HF), 10mg/kg of nano-phytosomes of Quercetin treated for 4 days (NQ), and 10mg/kg of nano-phytosomes of Quercetin + 2mg/kg of Hydroxychloroquine sulfate for 4 days (NQ+HF). Histo-pathological parameters and pro-inflammatory cytokines were evaluated.</span></span><span style="font-size:9.0pt"><span style="background:white"><span style="font-family:"Cambria",serif"></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span cambria="" style="font-family:"><span style="color:#7030a0">Findings:</span></span></span></b> <span style="font-size:9.0pt"><span cambria="" style="font-family:">Administration of <i>P. berghei</i> could increase the scores for histo-pathological parameters and levels of pro-inflammatory cytokines (p<0.0001). Administration of Hydroxychloroquine sulfate and nano-phytosomes of Quercetin could alleviate adverse effects of <i>P. berghei</i> on histo-pathological parameters (p<0.05).</span></span><span style="font-size:9.0pt"><span style="background:white"><span style="font-family:"Cambria",serif"></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:9.0pt"><span cambria="" style="font-family:"><span style="color:#7030a0">Conclusion:</span></span></span></b> <span style="font-size:9.0pt"><span cambria="" style="font-family:">A combination of nano-phytosomes of Quercetin and Hydroxychloroquine sulfate shows the best effect on treatment of <i>Plasmodium berghei </i>infections.<span style="background:white"></span></span></span></span></span></span><br> &nbsp; Malaria,Pro-inflammatory Cytokines,Nano-Phytosomes,Quercetin,Liver Damages, 19 23 http://gmedicine.de/browse.php?a_code=A-10-1-68&slc_lang=en&sid=2 M. Davoodi 10031947532846001252 10031947532846001252 No Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran F. Ahmed 10031947532846001253 10031947532846001253 No Livestock and Dairy Development Department Balochistan, Quetta, Pakistan M. Panizai 10031947532846001254 10031947532846001254 No Livestock and Dairy Development Department Balochistan, Quetta, Pakistan Z. Obeidavi 10031947532846001255 10031947532846001255 Yes Lorestan University of Medical Sciences, Khorramabad, Iran