en Original Research <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="background:white"><span cambria="" style="font-family:"><span style="color:#7030a0">Aims:</span></span></span></b> <span cambria="" style="font-family:">Non-alcoholic fatty liver disease has increased over the past years. It occurs when liver cells begin to collect fat droplets. Liver enzymes are the best indicator for assessing liver status. The incidence of this disease in women is higher than in men, although opposite results have been reported. The effect of mental and muscle relaxation in water was investigated to evaluate changes in liver enzymes, inflammatory markers, and C-reactive protein of Non-alcoholic fatty liver disease patients<span style="background:white">.</span></span><span style="background:white"><span style="font-family:"Cambria",serif"></span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="background:white"><span cambria="" style="font-family:"><span style="color:#7030a0">Materials & Methods:</span></span></span></b> <span cambria="" style="font-family:">This was a randomized clinical trial with a pretest-posttest and follow-up with the control group. Liver enzymes and inflammatory markers, including IL-6 and CRP, were measured before and after mental and muscle relaxation. The data was analyzed using an independent samples t-test, a paired t-test, and a two-way mixed ANOVA (repeated measures). Also, the correlation test was used to evaluate the correlation between age and FBS and liver enzymes.</span><span style="background:white"><span style="font-family:"Cambria",serif"></span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="background:white"><span cambria="" style="font-family:"><span style="color:#7030a0">Findings:</span></span></span></b> <span cambria="" style="font-family:">There were significant reductions in measured indices (FBS, AST, ALT, ALP, IL-6, and CRP) within the treatment group in comparison with the control group (p<0.05). Also, there were significant differences in means of AST (p<0.001), ALT (p<0.002), ALP (p=0.021), IL-6 (p<0.001), and CRP (p=0.019) between the two groups after mental and muscle relaxation in water<span style="background:white">.</span></span><span style="background:white"><span style="font-family:"Cambria",serif"></span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="background:white"><span cambria="" style="font-family:"><span style="color:#7030a0">Conclusion:</span></span></span></b> <span cambria="" style="font-family:">Mental and muscle relaxation in the water significantly improved and reduced the symptoms of NAFLD patients.<span style="background:white"></span></span></span></span></span><br> &nbsp; Nonalcoholic Fatty Liver Disease,Liver Enzymes,Interleukin 6,C Reactive Protein,Hydrotherapy, 57 61 http://gmedicine.de/browse.php?a_code=A-10-1-77&slc_lang=en&sid=2 N. Forghani 10031947532846001316 10031947532846001316 No Department of Cell and Molecular Biology, School of Biology, College of Sciences, Tehran University, Tehran, Iran M. Rahimian Boogar 10031947532846001317 10031947532846001317 No School of Medical, Shiraz University of Medical Sciences, Shiraz, Iran H. Faramarzi 10031947532846001318 10031947532846001318 Yes School of Medical, Shiraz University of Medical Sciences, Shiraz, Iran