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Volume 2, Issue 2 (2023)
GMJM 2023, 2(2): 63-67 |
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Received: 2022/11/2 | Accepted: 2023/03/1 | Published: 2023/04/24
Received: 2022/11/2 | Accepted: 2023/03/1 | Published: 2023/04/24
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Jazideh F, Tarkhnishvili E, Hashemi Feyzabadi S. Effects of Olea europaea L. Extract on Inflammatory Gene Expressions in Infected Wound Healing Process in Mice Model. GMJM 2023; 2 (2) :63-67
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1- Department of Medical Research, Institute for Intelligent Research, Tbilisi, Georgia
2- Semnan University of Medical Sciences, Semnan, Iran
2- Semnan University of Medical Sciences, Semnan, Iran
Abstract (453 Views)
Aims: Natural antimicrobial agents can decrease the risk of infection during wound healing. Olea europaea L. is an anti-inflammatory agent that can be used this way. This study was conducted for the first time to evaluate the effects of ointments prepared from olive leaf extract on inflammatory gene expressions in a mouse model's infected wound healing process.
Materials & Methods: We created two circular full-thickness wounds on the dorsal inter-scapular part of per mouse by a 5 mm biopsy punch and infected with Staphylococcus aureus and Pseudomonas aeruginosa. Following induction of infection, animals were treated with mupirocin (mupirocin group) and basal ointments containing 2.5% and 5% of the extract (2.5% & 5% OLE). A control group was also considered. We selected five mice per group, and samples were collected on days 3, 7, and 14 to investigate the total bacterial count and gene expressions.
Findings: The control group significantly showed higher wound area, total bacterial count, and higher expressions of IL-1β and TNF-α (p<0.05) and lower expressions for IL-10 and TGF-β (p<0.05). The treatment with OLE could significantly decrease wound area, total bacterial count, and expressions of IL-1β and TNF-α and increase the expression of IL-10 and TGF-β (p<0.05).
Conclusion: Ointments prepared from OLE could decrease total bacterial count, decrease the inflammatory phase, and improve wound healing.
Materials & Methods: We created two circular full-thickness wounds on the dorsal inter-scapular part of per mouse by a 5 mm biopsy punch and infected with Staphylococcus aureus and Pseudomonas aeruginosa. Following induction of infection, animals were treated with mupirocin (mupirocin group) and basal ointments containing 2.5% and 5% of the extract (2.5% & 5% OLE). A control group was also considered. We selected five mice per group, and samples were collected on days 3, 7, and 14 to investigate the total bacterial count and gene expressions.
Findings: The control group significantly showed higher wound area, total bacterial count, and higher expressions of IL-1β and TNF-α (p<0.05) and lower expressions for IL-10 and TGF-β (p<0.05). The treatment with OLE could significantly decrease wound area, total bacterial count, and expressions of IL-1β and TNF-α and increase the expression of IL-10 and TGF-β (p<0.05).
Conclusion: Ointments prepared from OLE could decrease total bacterial count, decrease the inflammatory phase, and improve wound healing.
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