Volume 3, Issue 1 (2024)                   GMJM 2024, 3(1): 19-23 | Back to browse issues page



How to cite this article
Davoodi M, Ahmed F, Panizai M, Obeidavi Z. Effect of Nano-Phytosome of Quercetin on Mice Liver Infected with Plasmodium berghei. GMJM 2024; 3 (1) :19-23
URL: http://gmedicine.de/article-2-245-en.html
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Rights and permissions
1- Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
2- Livestock and Dairy Development Department Balochistan, Quetta, Pakistan
3- Lorestan University of Medical Sciences, Khorramabad, Iran
* Corresponding Author Address: Lorestan University of Medical Sciences, Khorramabad, Iran. (ziaobd@gmail.com)
Abstract   (282 Views)
Aims: Malaria is one of most important diseases especially in tropical regions. Flavonoids are known to have beneficial properties that could be profitable. This study aimed to evaluate the effects of nano-phytosomes of Quercetin on liver damages of mice infected with Plasmodium berghei.
Materials & Methods: A total of 60 male BALB/c mice were intra-peritoneally infected by administration of 106 P. berghei-infected RBCs. Animals were acclimatized for 7 days and divided into 5 groups; 0.9% isotonic saline (as negative control), saline treated (as positive control), 2mg/kg of Hydroxychloroquine sulfate treated for 4 days (HF), 10mg/kg of nano-phytosomes of Quercetin treated for 4 days (NQ), and 10mg/kg of nano-phytosomes of Quercetin + 2mg/kg of Hydroxychloroquine sulfate for 4 days (NQ+HF). Histo-pathological parameters and pro-inflammatory cytokines were evaluated.
Findings: Administration of P. berghei could increase the scores for histo-pathological parameters and levels of pro-inflammatory cytokines (p<0.0001). Administration of Hydroxychloroquine sulfate and nano-phytosomes of Quercetin could alleviate adverse effects of P. berghei on histo-pathological parameters (p<0.05).
Conclusion: A combination of nano-phytosomes of Quercetin and Hydroxychloroquine sulfate shows the best effect on treatment of Plasmodium berghei infections.

1. World Health Organization. Antimicrobial resistance global report on surveillance: 2014 Summary [Internet]. Geneva: World Health Organization; 2014 [cited 2022 November 20]. Available from: https://apps.who.int/iris/bitstream/handle/10665/112647/WHO_HSE_PED_AIP_2014.2_eng.pdf;jsessionid=CADCEAFC2D8E52E06F32874A18A28CBE?sequence=1. [Link]
2. Reisinger EC, Fritzsche C, Krause R, Krejs GJ. Diarrhea caused by primarily non- gastrointestinal infections. Nat Clin Pract Gastroenterol Hepatol. 2005;2(5):216-22. [Link] [DOI:10.1038/ncpgasthep0167]
3. Vafaei MR, Kalani H, Pestehchian N, Nematolahi P. Histopathologic study on mice infected with Plasmodium berghei after treatment with sulfadoxine- pyrimethamine, pyrimethamine, and hydroxychloroquine sulfate. Compar Clin Pathol. 2017;27:123-9. [Link] [DOI:10.1007/s00580-017-2563-7]
4. Schofield L, Grau GE. Immunological processes in malaria pathogenesis. Nat Rev Immunol. 2005;5(9):722-35. [Link] [DOI:10.1038/nri1686]
5. Mahajan TMC, Chaudhari G. A novel approach towards phytosomal flavonoids. Pharm Sci. 2012;4:2079-121. [Link] [DOI:10.4103/0975-7406.94144]
6. Havsteen B. Flavonoids, a class of natural products of high pharmacological potency. Biochem Pharmacol. 1983;32(7):1141-8. [Link] [DOI:10.1016/0006-2952(83)90262-9]
7. Hollman PCH, Trijp JMP, Buysman M, Gaag M, Mengelers M, Vries J, et al. Relative bioavailability of the antioxidant flavonoid quercetin from various foods in man. FEBS Lett. 1997;418(1-2):152-6. [Link] [DOI:10.1016/S0014-5793(97)01367-7]
8. Guardia T, Rotelli AE, Juarez AO, Pelzer LE. Anti- inflammatory properties of plant flavonoids. Effects of rutin, quercetin and hesperidin on adjuvant arthritis in rat. Farmaco. 2001;56(9):683-7. [Link] [DOI:10.1016/S0014-827X(01)01111-9]
9. Vijayalakshmi A, Ravichandiran V, Velraj M, Nirmala S, Jayakumari S. Screening of flavonoid "quercetin" from the rhizome of Smilax china Linn. for anti- psoriatic activity. Asian Pac J Trop Biomed. 2012;2(4):269-75. [Link] [DOI:10.1016/S2221-1691(12)60021-5]
10. Wang L, Wang B, Li H, Lu H, Qiu F, Xiong L, et al. Quercetin, a flavonoid with anti-inflammatory activity, suppresses the development of abdominal aortic aneurysms in mice. Eur J Pharmacol. 2012;690(1-3):133-41. [Link] [DOI:10.1016/j.ejphar.2012.06.018]
11. Bose S, Michniak Kohn B. Preparation and characterization of lipid based nanosystems for topical delivery of quercetin. Eur J Pharm Sci. 2013;48(3):442-52. [Link] [DOI:10.1016/j.ejps.2012.12.005]
12. Li H, Zhao X, Ma Y, Zhai G, Li L, Lou H. Enhancement of gastrointestinal absorption of quercetin by solid lipid nanoparticles. J Control Release. 2009;133(3):238-44. [Link] [DOI:10.1016/j.jconrel.2008.10.002]
13. Liu D, Hu H, Lin Z, Chen D, Zhu Y, Hou S, et al. Quercetin deformable liposome: Preparation and efficacy against ultraviolet B induced skin damages in vitro and in vivo. J Photochem Photobiol B. 2013;127:8-17. [Link] [DOI:10.1016/j.jphotobiol.2013.07.014]
14. Xie Y, Luo H, Duan J, Hong C, Ma P, Li G, et al. Phytic acid enhances the oral absorption of isorhamnetin, quercetin, and kaempferol in total flavones of Hippophae rhamnoides L. Fitoterapia. 2014;93:216-25. [Link] [DOI:10.1016/j.fitote.2014.01.013]
15. Yang B, Chen F, Hua Y, Huang SS, Lin S, Wen L, et al. Prooxidant activities of quercetin, p- courmaric acid and their derivatives analysed by quantitative structure- activity relationship. Food Chem. 2012;131(3):508-12. [Link] [DOI:10.1016/j.foodchem.2011.09.014]
16. Saonere Suryawanshi JA. Phytosomes: An emerging trend in herbal drug treatment. J Med Genet Genomic. 2011;3(6):109-14. [Link]
17. Sharma L, Shukla G. Treatment of pregnant BALB/c mice with sulphadoxine pyrimethamine or chloroquine abrogates Plasmodium berghei induced placental pathology. Parasitol Int. 2014;63(1):49-56. [Link] [DOI:10.1016/j.parint.2013.08.016]
18. Satoskar AR, Simon GL, Hotez PJ, Tsuji M. Medical parasitology Austin. Florida: CRC Press; 2009. [Link] [DOI:10.1201/9781498713672]
19. Badri PN, Renu S. Role of medicinal plants in wound healing. Res J Med Plant. 2011;5(4):392-405. [Link] [DOI:10.3923/rjmp.2011.392.405]
20. Eo H, Lee HJ, Lim Y. Ameliorative effect of dietary genistein on diabetes induced hyper-inflammation and oxidative stress during early stage of wound healing in alloxan induced diabetic mice. Biochem Biophys Res Commun. 2016;478:1021-7. [Link] [DOI:10.1016/j.bbrc.2016.07.039]